Table 1 Antibodies and markers
Antigen | Host | Marker | Dilution | Supplier |
|---|---|---|---|---|
Biotinylated hyaluronan binding protein (bHABP) | Bovine | Hyaluronan | 1:200 | Associates of Cape Cod, Inc., East Falmouth, MA |
Cleaved caspase-3 (CC3) | Polyclonal Rabbit | Apoptotic cells | 1:500 | Cell Signaling Technology, Danvers, MA |
GFAP | Polyclonal Rabbit | Astrocytes | 1:500 | Dako North America, Inc., Carpinteria, CA |
Iba1 | Polyclonal rabbit | Microglia/macrophage | 1:500 | Wako Chemicals USA Inc., Richmond, VA |
Ki67 | Monoclonal mouse | Cell Cycle Activation | 1:200 | Novocastra, Buffalo Grove, IL |
MBP | Mouse | Mature OL | 1:500 | Covance, Princeton, NY |
O1 | Monoclonal mouse IgM | Immature/mature OL | 1:3000 | Dr. Rashmi Bansal (University of Connecticut Health Center, Farmington, CT) |
Biotinylated O4 | Monoclonal mouse IgM | Late OL progenitor/immature OL | 1:500 | Research Genetics, Huntsville, AL |
PDGFRα | Polyclonal rabbit | OL progenitor | 1:1000 | R&D Systems, Minneapolis, MN |
Olig2 | Mouse | Oligodendrocyte (OL) | 1:250 | Dr. John Alberta (Dana-Farber Cancer Institute, Boston, MA |