Skip to main content

Table 1 Antibodies and markers

From: An organotypic slice culture model of chronic white matter injury with maturation arrest of oligodendrocyte progenitors

Antigen Host Marker Dilution Supplier
Biotinylated hyaluronan binding protein (bHABP) Bovine Hyaluronan 1:200 Associates of Cape Cod, Inc., East Falmouth, MA
Cleaved caspase-3 (CC3) Polyclonal Rabbit Apoptotic cells 1:500 Cell Signaling Technology, Danvers, MA
GFAP Polyclonal Rabbit Astrocytes 1:500 Dako North America, Inc., Carpinteria, CA
Iba1 Polyclonal rabbit Microglia/macrophage 1:500 Wako Chemicals USA Inc., Richmond, VA
Ki67 Monoclonal mouse Cell Cycle Activation 1:200 Novocastra, Buffalo Grove, IL
MBP Mouse Mature OL 1:500 Covance, Princeton, NY
O1 Monoclonal mouse IgM Immature/mature OL 1:3000 Dr. Rashmi Bansal (University of Connecticut Health Center, Farmington, CT)
Biotinylated O4 Monoclonal mouse IgM Late OL progenitor/immature OL 1:500 Research Genetics, Huntsville, AL
PDGFRα Polyclonal rabbit OL progenitor 1:1000 R&D Systems, Minneapolis, MN
Olig2 Mouse Oligodendrocyte (OL) 1:250 Dr. John Alberta (Dana-Farber Cancer Institute, Boston, MA