ATBF1 protein expression level appears to decrease in age-dependent manner in wild-type mice. It increased in the brains of 17-month-old Tg2576 mice compared with age-matched wild-type mice. Lysates were prepared from whole brains of 10- and 17-month-old Tg2576 (n = 6) and age-matched wild-type (WT) mice (n = 6). The expression level of ATBF1 was determined by Western blot analysis using the anti-ATBF1 antibody. A, representative immunoblot is shown (upper panel), and bands were quantified by densitometry, normalized to actin, and expressed as a value relative to that in the 10-month-old WT mice as a control (lower graph). All the values are presented as mean ± SEM. *p < 0.05 vs 10-month-old WT mice (n = 6) and #p < 0.05 compared with those of 17-month-old WT mice, as determined by one-way ANOVA followed by Duncan's test.