Figure 4

DJ-1-specific action of DJ-1-binding compound-23. (A) Cell extracts were prepared from SH-SY5Y and KD-SH-SY5Y cells, and proteins in the extracts were analyzed by Western blotting with anti-DJ-1 and anti-actin antibodies. After membranes had been reacted with respective secondary antibodies, bands were visualized and their intensities were quantified using an infrared imaging system (Odyssey, LI-COR). (B and C) SH-SY5Y (B) and KD-SH-SY5Y (C) cells were treated with 1 μM of comp-23 for 20 hours and with various concentrations of H₂O₂ for 3 hours, and cell viability was measured by an MTT assay.