Figure 4

Flow cytometry analysis of blood monocyte subsets in WT, MyD88^-/-, APP _swe /PS1 and APP _swe /PS1-MyD88^+/- mice. Blood taken from the facial vein of 3-4 month-old mice was analyzed by FACS with the following fluorescently-labeled antibodies: CD45, CD11b, CD115 and Gr1. Leukocyte population is represented as cells in the CD45⁺ gate (a). To analyze exclusively monocytes in CD45⁺ cells, CD11b^hi and CD115^hi (CD45⁺CD11b⁺CD115⁺) cells were gated and the total percent for each group was quantified (b). No significant change in number of monocytes was revealed. Representatives histograms of monocytes for each group and their mean relative Gr1⁺ and Gr1^- values (c) showed significantly less Gr1⁺ and more Gr1^- monocytes in APP_swe/PS1-MyD88^+/- mice compare with APP_swe/PS1 mice. This difference is even more noticeable while expressing the ratio of Gr1⁺ on Gr1^- monocytes (d). Error bars: SEM; n = 3-5; *P < 0.05 (vs APP_swe/PS1). (Statistical analysis was performed using one-way ANOVA followed by a Dunnett's or Tamhane's post-hoc test).