BBC3 is important for the death of several retinal interneurons. Bbc3 and Bax deficiency increased the number of bipolar cells (VSX2+ cells) but not the number of horizontal cells (CALB1+ cells) in the inner nuclear layer (INL). The number of dopaminergic amacrine cells (TH), which reside in the INL were assessed in flat mount since there are so few in the retina. Deficiency in either Bbc3 or Bax greatly increased the number of these cells surviving in the adult retina. Quantification of VSX2, CALB1 and TH positive cells are given in table 1. Scale bar, 50 μm.