Figure 4
From: Upregulation of cathepsin D in the caudate nucleus of primates with experimental parkinsonism
Double immunofluorescence of CatD with MAP2 and IBA1 in CN od MPTP monkey. (A) Double immunostaining was performed on MPTP CN sections with the neuronal marker anti-MAP2 (red) and anti-Cat D (green). The merged image illustrates a co-localization of Cat D with MAP2, confirming its presence in neurons. Bar = 20 μm. Higher magnification of single neuron is also provided (bottom panel, Mag). Bar = 5 μm. (B) Double immunostaining was performed on MPTP CN sections with the microglial marker anti-IBA1 (red) and anti-Cat D (green). The merged staining of Cat D with IBA1 conforms the absence of Cat D in microglial cells. Bar = 20 μm. Higher magnification (bottom panel, Mag). Bar = 5 μm.