Light and confocal micrographs of different astrocytic phenotypes according to their enzyme content and cyoskeletal component in the hippocampus of 3xTg-AD mice. (A-B) Light microscopy images of GS (A) and GFAP (B) positive astrocytes showing their differential anatomical characteristics but similar domains. (C,E,G) Hippocampal confocal images evidencing astrocytic GS (C, red) and GFAP (E, green) expression pattern and their co-localisation (G, yellow). (D,F,H) High magnification confocal images illustrating the majoritary dual expression and co-existence of GS and GFAP (inserts D, F, H) in a representative astrocyte of the molecular layer of the DG. DG, dentate gyrus; GcL, granule cell layer; MoL, molecular layer; Lac, stratum lacunosum moleculare; Or, stratum oriens; PcL, pyramidal layer; Rad, stratum radiatum.