Accumulation of AD5 in rod cytoplasm analyzed in Tg(Rho:AD5:mCherry) larvae at different stages. (A) At 6 dpf, AD5:mCherry was exclusively localized to the nuclei of rods (arrows). (B) In contrast, AD5:mCherry was found also in the inner segments (arrowheads) of rods at 14 dpf. (C) Tg(Rho:Prpf31:mCherry) transgenic larvae at 14 dpf were used as control to show that Prpf31:mCherry is only found in rod nuclei (arrow) but not the cytoplasm. (D) In 21 dpf Tg(Rho:AD5:mCherry) larvae, AD5 protein as detected by PRPF31 antibody (green) was co-localized with mCherry (red) in both the nucleus (arrow) and rod inner segment (arrowhead). (E) In 21 dpf wild-type fish, endogenous Prpf31 protein was localized in all nuclei. Note that imaging exposure in E was extended compared to D to reveal nuclear localization of Prpf31 in all retinal cells. Shorter exposure in D indicates strong expression of transgenic AD5 under control of the rhodopsin promoter in rod photoreceptors.