Effect of SIN-1, paraquat and arginine on TDP-43 localization in SH-SY5Y cells. Cells were exposed overnight with 0.1 mM SIN-1, 1 mM paraquat or 1 mM arginine and TDP-43 localization was examined by immunofluorescence. A-C: untreated, D-F: SIN-1, G-I: paraquat, J-L: arginine. Green = TDP-43, blue = DAPI. Right-hand panel = merged images of TDP-43 and DAPI. Arrowheads show diffuse cytosolic TDP-43. Arrows show aggregated cytosolic TDP-43. Bar = 10 μm. Representative images from three separate experiments performed in duplicate or triplicate.