Figure 10
From: C-Jun N-terminal kinase controls TDP-43 accumulation in stress granules induced by oxidative stress
JNK inhibition blocks association of hnRNP K and TDP-43 with SGs. SH-SY5Y cells were treated with 1 mM paraquat overnight in the presence and absence of SP600125. A: Cells were immunoblotted for full length TDP-43 and 35 kDa CTF-TDP-43, B-Q: Cells were treated with paraquat and SP600125 and examined for TDP-43, hnRNP K or hnRNP A1 by immunofluorescence. B-E: untreated, labeled for TDP-43 (green) and hnRNP K (red); F-I: paraquat-treated, labeled for TDP-43 (green) and hnRNP K (red); J-M: paraquat and SP600125, labeled for TDP-43 (green) and hnRNP K (red); N-Q paraquat-treated, labeled for TDP-43 (green) and hnRNP A1 (red). Right-hand panel indicates merged images from TDP-43 and hnRNP panels. Arrows indicate SGs. Bar = 10 μm. Representative images from three separate experiments performed in duplicate or triplicate.