Figure 9
From: C-Jun N-terminal kinase controls TDP-43 accumulation in stress granules induced by oxidative stress
Aggregation of CTF-TDP-43 162-414 and 219-414 in SY5Y cells is partially associated with JNK. SH-SY5Y cells were transfected with GFP-tagged constructs for vector control (A-B), full length TDP-43 (C-D), CTF-TDP-43 219-414 (E-H) or CTF-TDP-43 162-414 (I-L). Green = TDP-43-GFP, blue indicates DAPI. Arrows indicate TDP-43 aggregates. Bar = 10 μm. Cells were transfected with CTF-TDP-43 162-414 or 219-414 for 24 hr and co-treated with SP600125 (JNK inhibitor) (G-H and K-L) or PD98059 (ERK inhibitor) (not shown) for a further 24 hr. The number of TDP-43-GFP aggregates per cell was determined (M). **p < 0.01. Representative images from two separate experiments performed in triplicate.