Angiotensin receptor expression in male and female rats. Real-time quantitative RT-PCR (A) and Western blot (WB; B) analysis of AT1, AT2 receptor expression in male rats as compared with female rats with estrogen (ovx+E2). Protein expression was obtained relative to the GAPDH band value and the expression of each gene was obtained relative to the housekeeping transcripts (β-Actin). The results were then normalized to ovx+E2 values (100%). Data are mean values ± SEM. *p < 0.05 (Student's t test).