Analysis of changes induced by treatment candesartan in male rats. Real-time quantitative RT-PCR (A) and Western blot (WB; B) analysis, and activity of the angiotensin converting enzyme (ACE; B) in male rats treated with candesartan. Blockage of AT1 receptors induced a significant increase in the expression of AT2 receptor and significant decrease in the expression of NADPH oxidase subunit p47phox in comparison with the corresponding untreated male rats; the activity of the angiotensin converting enzyme (ACE) was not significantly lower than in untreated rats. Protein expression was obtained relative to the GAPDH band value and the expression of each gene was obtained relative to the housekeeping transcripts (β-Actin). The results were then normalized to the values for control male rats (100%). Data are means ± SEM; *p < 0.05 (Student's t test).