Reduced toxicity of Aβ G37L expressed in C. elegans. A. Representative plot of paralysis onset in transgenic worms induced to express Aβ wild type (strain CL4176) or Aβ G37L (strain CL3523). In both of these strains the transgenes are marked with the dominant Roller mutation rol-6(su1006). B. Paralysis plot for an independent pair of transgenic strains induced to express Aβ wild type (CL2659) or Aβ G37L (CL2621). In both of these strains the transgenes are marked with the intestinal GFP marker Pmtl-2::GFP. C. Representative immunoblot of worm protein extracts (20 μg/lane) probed with anti-Aβ monoclonal antibody 6E10. Lane 1, 25 ng synthetic Aβ1-42; lane 2, CL4176 (Aβ wild type); lane 3, CL2659 (Aβ wild type); lane 4, CL3523 (Aβ G37L); lane 5, CL2621 (Aβ G37L). Note that although the overall patterns of oligomeric Aβ bands were similar in strains expressing Aβ wild type or Aβ G37L, differences did appear in the region of dimeric Aβ (arrow). D. Immunostaining with mAb 6E10 of transgenic worms expressing Aβ wild type (upper panel) or Aβ G37L (lower panel). Nuclei stained with DAPI (blue), 6E10 immunoreactivity in red. Size bar = 10 μm. E. Electron micrographs of sections of transgenic worms fixed by high pressure freezing. Left panel, section through body wall muscle of worm expressing Aβ wild type (CL4176). Note abnormal "light" mitochondria (arrows). Right panel, equivalent section through body wall of transgenic worm expressing Aβ G37L (CL3523). Note normal dark mitochondria (arrows). S = sarcomeres, N = nucleus, M = mitochondria. Size bar = 1 μm.