Figure 4
From: A glycine zipper motif mediates the formation of toxic β-amyloid oligomers in vitro and in vivo
Effect of G37L substitution on Aβ mediated aggregation and β-amyloid formation. A. Digital overlay of differential interference contrast (DIC) and epifluorescence images of live transgenic worms expressing GFP::Aβ fusion proteins. Top panel, third larval stage worm expressing GFP::Aβwild type fusion protein (strain CL1332). Note GFP inclusions in body wall muscle. Middle panel, third larval stage worm expressing GFP::Aβ L17P fusion protein (strain CL1364). Note diffuse GFP in body wall muscle. Lower panel, third larval stage worm expressing GFP::AβG37L fusion protein. Note similar GFP inclusions as observed in worms expressing GFP::Aβ wild type (top panel). Size bar = 100 μm. B. Amyloid formation in transgenic worms with constitutive muscle expression of Aβwild type or G37L. DIC/epifluorescence images of live adult transgenic worms stained with amyloid-specific dye X-34 (33). Top panel, CL2006 (wild type Aβexpression). Note lateral amyloid deposits (small arrows) in body wall muscle and non-specific staining of the buccal cavity (large arrow). Lower panel, CL2564 (AβG37L). Muscle X-34 deposits are undetectable, although staining is still seen in the buccal cavity (large arrow). Size bar = 100 μm. C. Quantification of X-34 deposits in CL2006 (Aβwild type), CL2564 (AβG37L), and CL802 (control non-transgenic) worms. Error bars = S.E.M.