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Figure 10 | Molecular Neurodegeneration

Figure 10

From: Phosphorylation of collapsin response mediator protein-2 disrupts neuronal maturation in a model of adult neurogenesis: Implications for neurodegenerative disorders

Figure 10

Increased CRMP2 phosphorylation in gp120 transgenic mice is rescued with down-modulation of CDK5 expression. Forty μm-thick vibratome sections from the brains of 8-month old non-transgenic (nontg), gp120 transgenic (tg), CDK5 heterozygous-deficient (CDK5+/-), and gp120 tg/CDK5+/- mice were processed for immunolabeling analysis with antibodies against CRMP2 and pSer522-CRMP2 (pSer-CRMP2). (A-D) Patterns of total CRMP2 immunoreactivity in the dentate gyrus from representative mouse brains showing a punctate expression pattern throughout the granular cell layer (GCL) and subgranular zone (SGZ), with background immunoreactivity detected in the molecular layer (ML). Intensely immunoreactive cell profiles were detected in the SGZ (arrows), particularly in the brains of gp120 tg mice. (E) Semi-quantitative image analysis of CRMP2 immunoreactivity in the hippocampal DG of nontg, gp120 tg, CDK5+/- and gp120 tg/CDK5+/- mice. (F-I) Patterns of pSer-CRMP2 immunoreactivity in the hippocampal DG from representative mouse brains showing a punctate expression pattern throughout the granular cell layer (GCL) and subgranular zone (SGZ), with background immunoreactivity detected in the molecular layer (ML). Intensely immunoreactive cell profiles were detected in the SGZ (arrows), particularly in the brains of gp120 tg mice. (J) Semi-quantitative image analysis of pSer-CRMP2 immunoreactivity in the hippocampus of nontg, gp120 tg, CDK5+/- and gp120 tg/CDK5+/- mice. * p < 0.05 compared to nontg controls by one-way ANOVA with post-hoc Dunnett's test (n = 4 per group). Scale bar = 30 μm.

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