Figure 2
From: Alzheimer-specific variants in the 3'UTR of Amyloid precursor protein affect microRNA function
SNPs are located near predicted miRNA binding sites on the APP 3'UTR and affect the function of miR-147 and miR-20a A. Schematic representation (not to scale) of SNP localization and predicted miRNA binding sites in the 3'UTR of hAPP. Red mutations represent the AD-specific SNPs, while the black mutations are not yet tested for their disease specificity. B. HEK293T cells were transfected with 5nM pre-miRs (as indicated) as well as a reporter construct containing the WT, T171C or A454G mutated 3'UTR of hAPP. The cells were lysed 24h post-transfection and luciferase signal was measured. After normalization for transfection efficiency, the signals were compared to the WT. Representative results (n = 3, performed in triplicate) are shown. Statistical significance was assessed by 2-way ANOVA (* = p < 0.05, ** = p < 0.01, *** = p < 0.001). SCR; scrambled, A.U.; Arbitrary Unit. C. Schematic representation of base pair matching between miRNAs and the 3'UTR of hAPP. The seed region of the miRNAs is indicated. The red bases represents the SNPs T171C (upper panel) or A454G (lower panel).