Preservation of cortico-striatal connectivity in R6/2:pGFAP-BDNF mice. Immunohistochemical staining against the pre-synaptic marker VGLUT1 (A) and the post-synaptic marker PSD-95 (B) was performed in 12-week-old wt, pGFAP-BDNF, R6/2 and R6/2:pGFAP-BDNF mice. The images were taken by confocal microscopy. VGLUT1- (A) and PSD-95- (B) positive particles were counted by the ImageJ software and represented in a graph as density of particles/field. Values are expressed as mean ± s.e.m. Data were analyzed by one-way ANOVA. * p < 0.05 and ** p < 0.01 as compared to wt mice; $ p < 0.05 and $$ p < 0.01 as compared to pGFAP-BDNF mice; # p < 0.05 as compared to R6/2 mice (n = 4/group). Scale bar 15 μm.