Figure 1
From: Expression of mutant TDP-43 induces neuronal dysfunction in transgenic mice
TDP-43 expression in TDP-43 M337V mice. (A) Western blots of brain lysates from non-transgenic (NT) mice, founder line 1, 4, 6 of TDP-43M337V hemizygous mice and line 3c of TDP-43WT hemizygous mice show that TDP-43M337V (lines 4, 6) and TDP-43WT mice are expression matched for both total (mTDP-43 and hTDP-43) and hTDP-43 levels, which was subsequently confirmed (B) by densitometric measurements. Compared with NT mice (1 ± 0.04), the levels of total TDP-43 in lines 1, 4, 6 and 3c are 1.2 ± 0.05; 1.6 ± 0.07; 1.3 ± 0.17; 1.9 ± 0.22 fold respectively. Compared to line 1(1 ± 0.06), the levels of hTDP-43 of line 4, 6 and 3c are 8.3 ± 1.2; 7.7 ± 1.0 and 8.3 ± 1.3 fold, respectively. Data shown in (B) are means ± SEM of six different mice of each genotype. (C) Western blot of tissue lysates of TDP-43 M337V mice from brain (Br), spinal cord (SC), thigh muscle (TM), heart (He), stomach (St), kidney (Ki), live (Li) and spleen (Sp) probed with an antibody against hTDP-43 reveals high expression in the brain and spinal cord (line 4). (D-E) Immunohistochemistry shows hTDP-43 distributed throughout the gray matter of the spinal cord (A) and brain (B) in the hemizygous TDP-43M337V mice.