Figure 2
From: Increased 90-kDa ribosomal S6 kinase (Rsk) activity is protective against mutant huntingtin toxicity
Overexpression of full-length mhtt in striatal cells increases Rsk levels. (A) Representative photomicrographs of htt and Rsk2 immunoreactivity in STHdhQ7/Q7 cells and striatal primary cultures after transfection (48 hours and 24 hours, respectively) with full-length wild-type (FL-17Q htt) or mutant (FL-75Q htt) htt. Closed arrows indicate transfected cells (cells expressing higher levels of htt) and open arrows indicate non-transfected cells. Scale bar: 16 μm. (B) Graphs showing Rsk1 and Rsk2 protein levels measured in STHdhQ7/Q7 cells, M213 cells and striatal primary cultures after transfection (48 hours for STHdhQ7/Q7 and M213 cells, and 24 hours for striatal primary cultures) with full-length wild-type (FL-17Q htt) or mutant (FL-75Q htt) htt. Data were expressed in arbitrary units and are the mean ± SEM of three independent experiments performed in triplicate. Data were analyzed by Student's t-test. *p < 0.05; **p < 0.01 and ***p < 0.001 as compared with FL-17Q htt-transfected cells.