Figure 6
From: Increased 90-kDa ribosomal S6 kinase (Rsk) activity is protective against mutant huntingtin toxicity
Inhibition of Rsk induces a reduction of pBAD and pSRF levels in STHdhQ111/Q111 cells. Lysates from STHdhQ7/Q7 and STHdhQ111/Q111 cells treated with or without BI-D1870 (0.1 μM; BI) were subjected to western blot to analyze pBAD (Ser-112)/Bad (A) and pSRF (Ser-103)/SRF (B) and tubulin protein levels. Results are expressed as the ratio between phospho and total Bad (A) and SRF (B) levels. Data are the mean ± SEM of four independent experiments performed in duplicate. Representative immunoblots are presented. Results are expressed in percentage respect to STHdhQ7/Q7 cells. Data were analyzed using two-way ANOVA followed by Bonferroni's post hoc test. *p < 0.05 and **p < 0.01 as compared with control STHdhQ7/Q7 cells and+++p < 0.001 as compared with control STHdhQ111/Q111 cells.