SEDI and C4F6 antibodies recognize diffusely distributed cytosolic mutant SOD1 in transiently transfected HEK293FT cells. Cells were transiently transfected with expression plasmids for WT (A-D) or A4V (E-H) SOD1 for 24 hours and stained with SEDI or C4F6 antibodies as explained in Methods. Pictures were taken with a 40× objective. Scale bars = 20 μm. Inset, image of rare cell with inclusion like structures. The images shown are representative of at least 3 independent transfection experiments.