Immunostaining of cells expressing SOD1::YFP fusion proteins with conformationally restricted SOD1 antibodies. HEK293FT were transfected with WT::YFP or A4V::YFP constructs, as noted on the Figure, and stained with hSOD1 (A-F), SEDI (G-L) or C4F6 (M-R) antibodies as explained in Methods. Pictures were taken using a 60× water immersion objective of a spinning disc confocal microscope, scale bars = 10 μm. Arrows indicate the location of SOD1::YFP inclusions and the absence of antibody staining. The images shown are representative of at least 3 independent transfection experiments.