Inclusions formed by mutant SOD1::YFP proteins are not released by saponin treatment. CHO cells were transiently transfected with expression plasmids for YFP, WT::YFP, and A4V::YFP as noted in the figure and then, after 24 hours, treated with saponin, fixed in 4% paraformaldehyde, stained with DAPI, and imaged. A, B, E, F, I, J, M, N, Q, R, and S were not treated with saponin. C, D, G, H, K, L, O, P, and T were treated with saponin. Scale bars in A-L are 50 μm. The images in M-T are digitally magnified cells from A-L. Because saponin completely extracted all cyotosolic fluorescence from cell expressing WT::YFP, no digital magnifications of these cells are shown. The images shown are representative of at least 3 independent transfection experiments. Scale bar = 50 μm. Images were capture on a standard fluorescence microscope.