GPX4 in nigral inclusions of PD brain. A. GPX4 expression (dark grey Ni-DAB, white arrowheads) coincides with AS-positive Lewy bodies (blue BCIP, marked by black arrowheads) in SN. Black arrows indicate neuromelanin. B. Confocal microscope images of two cells showing relationship of GPX4 (green) to AS (magenta). The above example shows some co-localization of GPX4 with AS within a Lewy body, while the below example shows GPX4 only around the Lewy body perimeter. Scale bars: A, 20 μm, B, 10 μm.