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Figure 11 | Molecular Neurodegeneration

Figure 11

From: Mitosis-specific phosphorylation of amyloid precursor protein at Threonine 668 leads to its altered processing and association with centrosomes

Figure 11

Centrosome association of Thr668 P-APP in mitotic cells: Asynchronously growing H4-APP cells were fixed and immunostained using Thr668 P-APP polyclonal and α-tubulin monoclonal antibodies and visualized using Alexa 594 (red) and 488 (green) fluorophores respectively. Nuclei were visualized using Hoechst staining. Staining was analyzed using the FV10-ASW 1.7 software for Olympus confocal microscope. Cells in metaphase and anaphase showed very clear P-APP localization at the centrosomes. Staining was very weak or absent in the interphase cells. Magnification: 63×.

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