Figure 5

Dantrolene feeding reduces NeuN-positive cell loss in YAC128 striata. (A) The brain weight of control (PBS-fed) and dantrolene-fed WT and YAC128 mice was measured at 14 months of age after 4% paraformaldehyde perfusion. The brain weight is shown as mean ± SE (g) (n = number of mice (10) in each groupsee Table 2). (B) The striatal slices from 14 months old control (PBS-fed) and dantrolene-fed WT and YAC128 mice were stained by the neuronal nuclear marker NeuN. Representative images are shown. (C) Striatal cell counts obtained as a result of stereological analysis of NeuN-stained striatal slices from 14 months old control (PBS-fed) and dantrolene-fed WT and YAC128 mice. The MSN numbers are shown as mean ± SE (n = number of mice, see Table 2). ***p < 0.001, *p < 0.05 using one-way ANOVA with Tukey's post test. WT, wild type; YAC, YAC128; Ctrl, control; Dan, dantrolene.