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Table 3 Targeted MS conditions for Septin11 peptides in gel band corresponding to 40-60 kDa.

From: Aberrant septin 11 is associated with sporadic frontotemporal lobar degeneration

   1Precursor Ions (m/z) 5Product Ions (m/z) 6AUC (FTLD-U) AUC (Control) FTLD-U/Control
Protein Name Peptide Sequences           
   Native (2L) 3IS (4H) Name Native (L) IS (H) (L) (H) (L) (H)  
Septin11 FESDPATHNEPGVR 778.4 783.4         
     y2-10 539.4 544.4 2420 1034 1776 1531 2.0
     y1-10 1077.3 1087.3 5428 1176 3548 1797 2.3
Septin11 AAAQLLQSQAQQSGAQQTK 979.0 983.0         
     y1-7 719.3 727.3 2700 790 1747 921 1.8
     y1-10 1046.4 1054.4 2242 743 1559 1030 2.0
     y1-12 1261.6 1269.6 3476 1074 1790 1334 2.4
            2.1 ± 0.2
   Precursor Ions ( m/z )     7 XIC (FTLD-U) XIC (Control)  
   Native (L) IS (H)     (L) (H) (L) (H)  
β-actin AGFAGDDAPR 488.73 493.74     3208 916 3799 717 0.7
β-actin GYSFTTTAER 566.77 571.78     2651 740 4109 896 0.8
β-actin SYELPDGQVITIGNER 895.96 900.96     2474 704 2806 584 0.7
            0.7 ± 0.1
  1. 1The m/z values of native and labeled peptides 2Light 3Internal Standard 4Heavy 5The name and m/z values of monitored product ions (e.g. y1-7, the singly-charged y7 ion) 6Area under the curve 7Extracted ion chromatogram.