Table 3 Targeted MS conditions for Septin11 peptides in gel band corresponding to 40-60 kDa.
From: Aberrant septin 11 is associated with sporadic frontotemporal lobar degeneration
| Â | Â | 1Precursor Ions (m/z) | 5Product Ions (m/z) | 6AUC (FTLD-U) | AUC (Control) | FTLD-U/Control | |||||
|---|---|---|---|---|---|---|---|---|---|---|---|
Protein Name | Peptide Sequences | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â |
| Â | Â | Native (2L) | 3IS (4H) | Name | Native (L) | IS (H) | (L) | (H) | (L) | (H) | Â |
Septin11 | FESDPATHNEPGVR | 778.4 | 783.4 | Â | Â | Â | Â | Â | Â | Â | Â |
| Â | Â | Â | Â | y2-10 | 539.4 | 544.4 | 2420 | 1034 | 1776 | 1531 | 2.0 |
| Â | Â | Â | Â | y1-10 | 1077.3 | 1087.3 | 5428 | 1176 | 3548 | 1797 | 2.3 |
Septin11 | AAAQLLQSQAQQSGAQQTK | 979.0 | 983.0 | Â | Â | Â | Â | Â | Â | Â | Â |
| Â | Â | Â | Â | y1-7 | 719.3 | 727.3 | 2700 | 790 | 1747 | 921 | 1.8 |
| Â | Â | Â | Â | y1-10 | 1046.4 | 1054.4 | 2242 | 743 | 1559 | 1030 | 2.0 |
| Â | Â | Â | Â | y1-12 | 1261.6 | 1269.6 | 3476 | 1074 | 1790 | 1334 | 2.4 |
|  |  |  |  |  |  |  |  |  |  |  | 2.1 ± 0.2 |
| Â | Â | Precursor Ions ( m/z ) | Â | Â | Â | 7 XIC (FTLD-U) | XIC (Control) | Â | |||
| Â | Â | Native (L) | IS (H) | Â | Â | Â | (L) | (H) | (L) | (H) | Â |
β-actin | AGFAGDDAPR | 488.73 | 493.74 |  |  |  | 3208 | 916 | 3799 | 717 | 0.7 |
β-actin | GYSFTTTAER | 566.77 | 571.78 |  |  |  | 2651 | 740 | 4109 | 896 | 0.8 |
β-actin | SYELPDGQVITIGNER | 895.96 | 900.96 |  |  |  | 2474 | 704 | 2806 | 584 | 0.7 |
|  |  |  |  |  |  |  |  |  |  |  | 0.7 ± 0.1 |