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Figure 1 | Molecular Neurodegeneration

Figure 1

From: Analysis of striatal transcriptome in mice overexpressing human wild-type alpha-synuclein supports synaptic dysfunction and suggests mechanisms of neuroprotection for striatal neurons

Figure 1

Corroboration of microarray results for differentially expressed genes. Confirmation of the changes of selected microarray differentially expressed genes by comparison to the changes assessed by quantitative real-time polymerase chain reaction (qRT-PCR) using: A- pooled RNA from wild-type (wt) and α-synuclein (SNCA) overexpressing transgenic (tg) mice groups; B- RNA from individual wt and tg mice. The qRT-PCR average fold changes of selected genes in tg mice striata normalized to Gapdh (Glyceraldehyde-3-phosphate dehydrogenase), and relative to expression in wt mice (dotted line) are shown (mean ± SEM); ns (not significantly different expression between tg and wt mice, using a t-test, n = 3, p > 0.05); SEM values in (A) show qRT-PCR technical variation in, and in (B) show variation between mice plus qRT-PCR technical variation). Pearson's test found significant correlation between microarray and qRT-PCR results in A (r2 = 0.7288, p < 0.0001) and B (r2 = 0.7354, p < 0.0001). Ttr (transthyretin), Phgdh (3-phosphoglycerate dehydrogenase), Pde7b (phosphodiesterases 7b), Tnn1 (troponin t1), Adora2a (adenosine A2a receptor), Meg3 (Maternally expressed 3), Drd2 (dopamine receptor 2), Rasd2 (rasD family, member 2), Psmb6 (proteasome (macropain) subunit, β 6), Med1 (mediator complex subunit), Ptprk (protein tyrosine phosphatase, receptor, K), Pttg1 (pituitary tumor-transforming 1), Nov (Nephroblastoma Overexpressed), Pparbp (peroxisome proliferator activated receptor binding protein), Stx1a (syntaxin 1a), Nr4a2, Psmc4 (proteasome (macropain) 26S subunit, ATPase, 4), Mef2c (myocyte enhancer factor 2c), Cckbr (cholecystokinin B receptor), Dhcr24 (24-dehydrocholesterol reductase), Ckmt1 (creatine kinase, mitochondrial 1), Srebf2 (sterol regulatory element-binding factor 2), Trh (thyrotropin-releasing hormone), Bdnf (brain derived growth factor), Gpx3 (glutathione peroxidase 3), Ywhag (Y-3-monooxygenase/W 5-monooxygenase activation protein, γ polypeptide).

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