Figure 2
Validation of microarray and qRT-PCR results for the highly expressed transthyretin ( Ttr ) gene. The confirmation of the differential expression of Ttr was achieved by measuring striatum Ttr protein levels by ELISA in individual wt (wild-type) mice and aso (α-synuclein (SNCA) overexpressing or Thy1-aSyn mice). A- The average Ttr protein levels in the striata from Thy1-aSyn mice and wt mice groups are shown (mean + SEM, **p < 0.01, non-parametric Mann-Whitney t-test, aso vs wt, n = 3 per group); levels of Ttr in each individual mouse are also shown to illustrate variation between mice (error bars represent SEM showing technical variations in triplicate ELISA measurements). B- The average human SNCA mRNA levels in the striata from Thy1-aSyn mice and wt mice groups determined by qRT-PCR, normalized to Gapdh, and relative to the levels of endogenous Snca mRNA in the wt mice that partially hybridize to the human SNCA primers are shown (mean + SEM, ***p < 0.001, non-parametric Mann-Whitney t-test, aso vs wt, n = 3 per group); levels of SNCA mRNA in each individual mouse are also shown to illustrate variation between mice (error bars represent SEM showing technical variation in triplicate qRT-PCR analyses. Pearson's test found significant correlation (r2 = 0.6791, p < 0.043) between the two measured expression values in A and B.