Analysis of striatal transcriptome in mice overexpressing human wild-type alpha-synuclein supports synaptic dysfunction and suggests mechanisms of neuroprotection for striatal neurons

Table 1 Array data quality control and differentially-regulated genes in ASO mice

Mice Genotype	SNCA expression		Data quality control			Altered genes
	species	fold change (mean ± sd)	source	% calls (present)	intensity (mean ± sd)	up	down	total
ASO	m *Snca* ^a	0.9 ± 0.1	All ^c	57.5	197 ± 445	349	484	833
	h *SNCA* ^b	155 ± 85	D.E.G. ^d	84.1	220 ± 271	96	137	233
wt	h *SNCA*	1.1 ± 0.5	All ^c	58.6	194 ± 425	0	0	0
	m *Snca*	1.0 ± 0.0	D.E.G. ^d	91.3	223 ± 295	0	0	0

Abbreviations: ASO: α-Synuclein (SNCA) overexpressing; wt; wild type; D.E.G.: differentially expressed gene
Notes: a- mSnca detected by 3 probe sets in theMoe-430A chip; b- hSNCA measured by qRT-PCR in 3 wt and 3 ASO mice (Figure 2B); c- Include all changed genes P < 0.005; d- Selection criteria for D.E.G. (1) gene is present in at least one of the mice groups examined and (2) Fold change > 1.52 (Signal log2 ratio > 0.6), p < 0.005.

ISSN: 1750-1326