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Figure 1 | Molecular Neurodegeneration

Figure 1

From: Resorufin analogs preferentially bind cerebrovascular amyloid: potential use as imaging ligands for cerebral amyloid angiopathy

Figure 1

Resorufin preferentially binds CAA in aged Tg2576 brain sections. Paraformaldehyde-fixed brain sections prepared from 16-month-old Tg2576 transgenic mice (Tg) or littermate wild-type mice were co-stained with 1 μM resorufin (Res) and 2 μM methoxy-X34 (X34), followed by fluorescent microscopy (N = 6-8). Representative images of X34 and resorufin staining in Tg2576 mice (A-C) and wild-type mice (D) are shown. Resorufin selectively binds CAA-laden arterioles (arrowheads) but not senile plaques (arrows), whereas the Congo red derivative methoxy-X34 labels both. Both resorufin and methoxy-X34 staining was absent in age-matched WT mice. Scale bars in A-D: 100 μm. CAA loads (E) and neuritic plaque loads (F) as determined by methoxy-X34 (X34) staining vs. resorufin staining in the motor cortex of Tg2576 mice were plotted.

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