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Figure 2 | Molecular Neurodegeneration

Figure 2

From: Resorufin analogs preferentially bind cerebrovascular amyloid: potential use as imaging ligands for cerebral amyloid angiopathy

Figure 2

Multi-photon imaging reveals colocalization of resorufin and methoxy-X34 in CAA-affected vessels. Fixed whole brains prepared from aged Tg2576 mice were labeled with a vascular smooth muscle cell (VSMC) marker phalloidin-Alexa 488 (green), and amyloid binding dyes methoxy-X34 (blue) and resorufin (red), followed by imaging by multi-photon microscopy (n = 7). A. Representative fluorescent images in the same field demonstrate co-localization of resorufin and X34 staining in the leptomeningeal vessels of aged Tg2576 mice. B. Higher magnification images show that both resorufin and methoxy-X34 are co-localized to the VSMC layer of the vessel segment having severe disruption of VSMC arrangement - a characteristic feature of CAA. In contrast, both methoxy-X34 and resorufin staining is absent in the vessel segment with no CAA pathology. Scale bars, in A: 50 μm; in B: 25 μm.

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Molecular Neurodegeneration

ISSN: 1750-1326