Figure 7

Gliosis in Hup23 Tg mouse brain. A, Sagittal brain sections of Hup23 Tg mice and their Ntg littermates were stained with an antibody against GFAP. A pair of representative images is shown at lower magnification. B, Higher magnification images of cortex (Cx), superior colliculus (SC), deep cerebellar nuclei (DCN), and brainstem (Bs) [regions indicated by boxes in panel A] are shown. C, Quantification of astrogliosis in Hup23 brainstem. The number of star-shaped GFAP positive astrocytes was counted in four defined regions within brainstem (see the schematics on the left) in Hup23 mice and littermates from 4 different lines, and the mean ± SEM is plotted. Pair wise t-test ***, p < 0.001, ** p < 0.01, * p < 0.05.