Figure 2

AAV1/2-5LO over-expression modulates brain Aβ peptides levels and deposition in Tg2576 mice. A. RIPA-soluble (left panels) and formic acid extractable (right panels) Aβ1-40 and Aβ1-42 levels in cortex of Tg2576 receiving empty vector (CTL) or AAV1/2-5LO gene were measured by sandwich ELISA. (n = 8 for CTL, and n = 10 for AAV-5LO; *p < 0.05). B. Representative sections of brains from Tg2576 mice receiving AAV1/2-5LO or empty vector (CTL) immunostained with 4G8 antibody. C. Quantification of the area occupied by Aβ immunoreactivity in brain of Tg2576 mice receiving AAV1/2-5LO or empty vector (CTL) (*p < 0.05). Values represent mean ± SEM (n-8 CTL; n = 10 AAV-5LO).