Figure 3
AAV1/2-5LO over-expression alters brain APP metabolism via the γ-secretase pathway in Tg2576 mice. A. Representative western blots of 5LO, APP, ADAM-10, BACE-1, sAPPα, sAPPβ, CTFα, CTF-β, PS1, Nicastrin, APH-1, Pen-2, apoE, neprilysin, IDE in brain homogenates from Tg2576 mice receiving AAV1/2-5LO or empty vector (CTL). B. Densitometric analyses of the immunoreactivities to the antibodies shown in the previous panel. Values represent mean ± SEM (* p < 0.01). C. Representative sections of different brain regions from Tg2576 mice receiving AAV1/2-5LO or empty vector (CTL) immunostained for 5-LO (× 20 magnification). D. Correlation between levels of 5LO protein and RIPA soluble Aβ 1-40 peptides (r2 = 0.61, p = 0.0003). E. Representative brain sections from Tg2576 receiving AAV1/2-5LO or empty vector (CTL) immunostained for GFAP and CD45 (× 20 magnification). F. Quantitative analysis of the immunoreactivity for GFAP and CD45 in the same animals.(*p < 0.004).