Figure 2

Impaired locomotive ability of I2020T LRRK2 TG mice. (A) Beam test. Each mouse was forced to walk on a narrow square beam (100 cm long, 5 mm wide). The time taken, the number of steps required to reach the platform, and the frequency of slips, were recorded. Left: number of slips normalized by time spent. Right: number of slips normalized by total steps: 23 weeks (NTG, n=13; TG, n=11), 43 weeks (NTG, n=13; TG, n=11), 73 weeks (NTG, n=6; TG, n=9). (B) Rotarod test. The time until the mouse fell from the rotating rod (16 rpm, 3 cm diameter) was recorded. The averages of three trials in one day are shown. The test was continued for 5 days (NTG, n=14; TG, n=11; 34 weeks). (C) Cylinder test. The frequency of rearing in the cylinder (9.5 cm diameter) during 20 min is shown (NTG, n=13; TG, n=11; 22 weeks). In all graphical representations, data are expressed as mean ± SEM and were assessed by Student’s t test at each time point; * p<0.05. ** p<0.01.