Figure 6

Vulnerability and defective neurites of primary TH⁺neurons derived from I2020T LRRK2 TG brain. (A) Anti-TH immunostaining of primary neurons prepared from the midbrain of I2020T LRRK2 TG and NTG mice. Apoptotic neurons were visualized by TUNEL assay. Scale bar: 30 μm. (B) Ratio of TUNEL-positive cells among TH⁺ neurons during culture. (C) Anti-TH immunostaining of primary neurons and the tracing image of neurites. Scale bar: 30 μm. (D) Left: number of neurite branches of TUNEL-negative TH⁺ neurons. Right: total neurite outgrowth of TUNEL-negative TH⁺ neurons (NTG, n=15; TG, n=15). Quantitative data are expressed as mean ± SEM and were assessed by Student’s t test at each time point; *p<0.01.