Figure 1

MoRK13 and MoRK13-inf PrP localises predominantly to lipid raft fractions. Representative immunoblots of subcellular fractions (1-10) obtained from MoRK13 and MoRK13-inf. Equivalent volumes of each fraction were resolved on gradient gels. Proteins were transferred to PVDF, which was sectioned based on the predicted molecular weight of the protein of interest, with each PVDF strip probed with the relevant antibody. CTB dot blot - 3 μl of fraction was dried onto nitrocellulose membrane, and blotted as described in the methods. LR = lipid raft; ER = endoplasmic reticulum; MT = mitochondria; EE = early endosome; CTB = cholera toxin subunit B (LR marker); Flot1 = Flotillin 1 (LR marker); Bcl-2 = anti-apoptotic MT marker protein; Bip = ER lumen chaperone protein; EEA1 = EE antigen 1.