Figure 3

All MoRK13-inf fractions contain infectivity. (A) Representative cell blot showing PrP^res levels produced by recipient MoRK13 cells at passage 4 (P4) post-exposure to equal volumes of each fraction at the 'neat' dilution (N) or further serially diluted (1/10-1/1000). (B) Quantification of infectivity from three independent MoRK13-inf fractionations (n = 3); fractions enriched in lipid raft (LR), endoplasmic reticulum (ER), mitochondrial (MT) and early endosome (EE) marker proteins are highlighted. Statistical analysis by one way ANOVA; only the statistical findings for relevant comparisons are indicated: *** p < 0.001, ** p < 0.001, * p < 0.05, ns = not significant.