Figure 5
Lesion profiles from Tga20 mice intracerebrally inoculated with M1000, MoRK13 and MoRK13-inf whole cell lysates and selected MoRK13-inf fractions. Lesion profiles generated by quantification of the degree of vacuolation, reactive astrocytosis and PrP plaques in each brain region (HI - hippocampus, TH - thalamus, OP - occipital pole, CE - cerebellum, MB - midbrain, PO - pons) in Tga20 mice inoculated as indicated. n = 3 or 4 for all brain regions except in MoRK13-inf whole lysate HI H&E and 0.01% M1000 HI PrP plaque deposition, where n = 2. Statistical analysis comparing lesion profiles was by two way ANOVA. The only significant difference was comparing MoRK13-inf and Fraction #10 in OP region (*p < 0.05).