Figure 4
Inhibition of γ-secretase causes an accumulation of β-CTF and α-CTF in hippocampal synaptic preparations. a. Hippocampal synaptosomes preparations from 4 FDDKI mice treated with GSI for 5 hrs and 4 FDDKI vehicle-treated animals (Control) were analyzed by WB for APP and CTFs. b. Graph representing quantization of quadruplicate samples. The levels of β-CTF and α-CTF in vehicle-treated mice were assigned an arbitrary value of 100. The amounts of β-CTF and α-CTF in treated samples were expressed as a % of the levels in the control. Synaptic fractions from GSI treated FDDKI mice express significantly more α-CTF (P=0.0004) and β-CTF (P=0.0047) than vehicle-treated samples. Similar levels of mAPP are present in both groups. c. The α-CTF/mAPP and β-CTF/mAPP ratios are increased in treated mice compared to vehicle-treated samples (P=0.035 and P=0.02, respectively. d. Synaptic fractions from both GSI treated and vehicle-treated FDDKI mice express similar levels of mBri2. e. Quantization of mBRI2 levels in quadruplicate samples.