Morphological and histological analyses of LRRK2 -/- kidneys at various ages. A. Initial discoloration, enlarged size, and altered granular tissue texture are observed in kidneys of male LRRK2-/- mice beginning at 3-4 months of age, the extent of abnormalities increases with age. B. The ratio of kidney/body weight is significantly increased in male LRRK2-/- mice at the ages of 1, 4, and 7 months but decreased drastically at 20 months of age. C. Autofluorescence in the epithelial cells of the proximal tubules of the renal cortex of LRRK2-/- mice. Autofluorescent intracellular granules are more visible in the epithelial cells of the proximal tubules of LRRK2-/- kidneys at 7 months than 4 months of age. By 20 months of age, the cytosolic regions of the epithelial cells of the proximal tubules in the LRRK2-/- kidneys are occupied by large but irregular structures showing strong autofluorescence. DAPI-stained nuclei are in blue. All scale bars: 20 μm.