Figure 6

SB-3CT attenuates gelatinase-associated loss of endothethial cells and decreases ICH. a Representative brain sections stained with cresyl violet reveal secondary micro-hemorrhages in the ischemic cortex after embolic stroke in mice. Top panels: A lower magnification of serial sections, which are separated in 960 μm intervals for adjacent sections. Lower panels: Higher magnification of the cresyl violet-stained brain sections (separated in 200 μm intervals for two adjacent sections), in the ischemic cortex 1 day after treatment with either vehicle (a-d) or SB-3CT (a’-d’). Scale bar, 300 μm. b Quantification of hemorrhagic volume. Micro-hemorrhages were evaluated in cresyl violet-stained brain sections by bright-field microscopy. ICH volumes were quantified using the stereology technique, which utilizes systematic sampling of 25–30 serial sections per brain, each section separated by 200-mm along the anteroposterior axis of the mouse brain. Analysis of the ICH volumes revealed that SB-3CT significantly reduced ICH volume in ischemic brains (n = 3), p < 0.05 by one-tailed Student’s t-test, and data are expressed as means ± SEM. c Fluorescence staining shows loss of lectin-positive endothelial cells in the embolic ischemic cortex. SB-3CT blocks deformation of endothelial cells in the ischemic cortex. Scale bar, 50 μm.