Figure 8

Unaltered mitochondrial morphology in PINK1 −/− MEFs and neurons. A. Distribution of live PINK1−/− and +/+ MEFs infected with Mt-DsRed to the four morphological categories I, II, III and IV. B. Distribution of fixed PINK1−/− and +/+ MEFs stained with MitoTracker Red (250 nM) to the four morphological categories. C. Distribution of fixed PINK1−/− and +/+ cortical neurons stained with MitoTracker Red (100 nM) to the four morphological categories. D. Representative fluorescent images of mitochondria stained with MitoTracker Red (250 nM) i) and the resulting binary (ii) used for quantitative analysis. E. Quantitative analysis using ImageJ shows the size and the number of branches of mitochondria in PINK1−/− and +/+ MEFs (P > 0.05 for all parameters measured). All data are expressed as mean ± SEM. The numbers shown indicate the number of independent experiments performed in the study. For each experiment 100 cells were analyzed quantitatively.