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Figure 9 | Molecular Neurodegeneration

Figure 9

From: Regulation of mitochondrial permeability transition pore by PINK1

Figure 9

Higher mitochondrial calcium levels in PINK1 −/− cells. A. Representative Fura-2 images of Ca2+ responses following FCCP treatment in PINK1−/− and +/+ MEFs. Fura-2 ratios at 340/387 are shown at time points indicated. The pseudocolor calibration scale for 340/387 ratios is shown on the right. FCCP (1 μM) was added at t = 25 s. B. Left panel: Time course of cytosolic [Ca2+] rise following FCCP treatment in PINK1−/− and +/+ MEFs. Middle panel: The peak value of cytosolic calcium rise following FCCP is higher in PINK1−/− cells. Right panel: The average size of the FCCP releasable Ca2+ pool is higher in PINK1−/− MEFs compared to controls. C. Representative Fura-2 images of Ca2+ responses following FCCP treatment in PINK1−/− and +/+ neurons. D. Left panel: Time course of cytosolic [Ca2+] rise following FCCP treatment in PINK1−/− and +/+ neurons. Middle panel: The peak value of cytosolic calcium rise following FCCP is higher in PINK1−/− neurons. Right panel: The average size of the FCCP releasable Ca2+ pool is higher in PINK1−/− neurons. All data are expressed as mean ± SEM. ** p < 0.01.

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