Expression of D1 receptor, mGluR5, and glutamate receptor subunits. (A) Representatives of western blot from the prefrontal cortex of Fmr1 WT and KO mice. (B) There were no differences in expressions of D1 receptor, mGluR5, NR2A, and NR2B between the WT and Fmr1 KO mice ACC. n = 4 mice per group. (C) Representatives of western blot to detect the level of phosphorylation of NR2B subunit at Tyr-1472 (p-NR2B-Tyr1472). (D) Level of p-NR2B-Tyr1472 was increased after treatment with SKF81297 (5 μM) alone or combining with DL-AP3 (10 μM) in neurons from Fmr1 WT and KO mice. n = 4 dishes. * p < 0.05, ** p < 0.01 compared with control; # p < 0.05 compared between Fmr1 WT and KO mice.