Quantitative differences in p62 levels and LC3-II/LC3-I ratio in aged LRRK2 KO mice. (a) Representative LC3 and p62 immunoblots of insoluble fraction kidney lysates from 3-, 12- and 20-month old WT and KO mice. Visibly more p62 is seen in both 12- and 20-month old mice. LC3-II levels are also visibly increased in 20-month old KO mice. (b) Graphical presentation of densitometric quantification of immunoblots from N = 5-7 animals for each group. Alzheimer (AD) brain lysate was used as a control. LC3 data are expressed as a ratio of LC3-II/LC3-I. The ratio of LC3-II/LC3-I was significantly increased by 20 months, indicating an increase in autophagy. p62 data was expressed as % intensity, normalized to the lowest densitometric band on each blot. A significant increase was observed in KO mice at 12 and 20 months. Data are presented as mean ± SEM and were analyzed by either Student’s t-test or Mann Whitney non-parametric comparisons. ** p <0.01, ***p <0.001.