SNX12 regulates Aβ generation. HEK-APPSwe cells were transfected with (A) SNX12 and control plasmids, or (B) two SNX12 siRNAs (1 and 2) and a control scrambled siRNA. Aβ in conditioned media and total APP and SNX12 in cell lysates were analyzed by Western blot. The levels of Aβ were quantified by densitometry for comparison. N = 3, *: p < 0.05. (C) Primary neurons derived from APP/PS1/tau triple transgenic mice at postnatal day 0 were infected with SNX12 shRNA or control lentiviral particles for 72 h. Neuron lysates were analyzed for APP, PS1-NTF, BACE1, SNX12 and MAP2 by Western blot. Secreted Aβ40 and Aβ42 in conditioned media were quantified by ELISA. The levels of Aβ40 and Aβ42 were normalized to those of controls (set as one arbitrary units) for comparison. N = 3, *: p < 0.05.