Figure 2

SNX12 is abundantly expressed in mouse brain tissues and localized in the early endosomes. The expression of SNX12 in various (A) tissues and (B) brain regions of two month old C57/Bl6 mice were analyzed by Western blot. (C) SNX12-EGFP was transfected into HeLa cells. Cells were fixed, permeabilized, and incubated with antibodies against the early endosome marker EEA1 or the late endosome marker cd63. After additional incubation with Alexa 594-conjugated secondary antibody, cells were examined with a fluorescence confocal microscope. Green: SNX12; red: EEA1 or cd63.